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Micropipette Calibration Tips

Special pipette tips with fine circuit traces used to calibrate pipette by measuring electrical resistance
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Micropipettes are routinely used by molecular biologists, organic chemists, and other bench scientists to deliver precise volumes of liquid on the microlitre to millilitre scale. On occasion, they must be calibrated to verify their accuracy. This is typically done by pipetting a volume of water on to an analytical balance, measuring the weight of the water, and then using the density of the water at room temperature to calculate the volume delivered. This works fine and dandy for volumes greater than fifty microlitres or so, but below that, the weights of the microdroplets become difficult to measure.

My solution to this problem is the development of a specialized micropipette tip that measures the volume of liquid taken up by the pipette. On the inside of this tip are printed extraordinarily fine circuit traces that measure the level of the special, conductive testing fluid. The traces that are exposed to the fluid are arrange radially so that a "slice" of fluid will close the circuit at the next measurement level. One may need to use several different tips to calibrate over the range of the pipette. For example, a 10 uL pipette may have three different tips for calibration at 1 uL, 5 uL, and 10 uL.

The base of the tip "plugs in" to a battery operated, wristwatch-sized computer with an LCD screen that displays the volume delivered and calculates the error. The tip is then flushed, dried, and stored for reuse at a later date.

I don't know how you would print circuit traces on the inside of the tip, but I suppose there's someone out there who could figure it out.

Cuit_au_Four, Jun 20 2013

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       Why do micropipettes need to be recalibrated ? Does their volume change somehow ?
FlyingToaster, Jun 20 2013
  

       If they're adjustable (as most are), the adjuster can slip out of calibration. However, 99% of pipette faults are due to leaky pistons, which can be checked much more easily (fill pipette; place finger over end; depress plunger and wait 5 seconds; remove finger; if liquid isn't expelled, there's a leak).   

       This is a nice idea, though. My only quibble is that, especially if the inside of the tip isn't uniform or is slightly dirty, the liquid/air interface may not be perfectly level, leading to an over- or under-reading. But using a special tip which is very long and narrow would solve this problem.
MaxwellBuchanan, Jun 20 2013
  

       my assumption would be that an accurate TD measure is not achieved until the tip has been filled with fluid.   

       how does one address the strange behavior of fluid at that volume? The meniscus is a dynamic thing and tiny droplets of fluid are capricious and very responsive to surface conditions, even static. I'm afraid that I would have very little trust that my investment in such a device would be worthwhile. After all, I needn't measure the mass of a single delivery, I can measure more accurately the mass of 10, 20, 50, 100 deliveries in bulk and do some math which should give adequate significance given the vagaries affecting my accuracy (thermometer and scale accuracy and resolution).
WcW, Jun 20 2013
  

       TD accuracy is always a little hit and miss. I assume that the values are different if the tip is submerged, and don't even get started with multiwell tips.
WcW, Jun 20 2013
  

       To be honest, I can't think of a lot of situations where even a 5% pipetting error is a big deal. And there are very few where a 1% error is worth worrying about.   

       You are more likely to get larger errors through bad pipetting technique; failing to mix defrosted solutions before sampling; failing to correctly touch off the tip after dispensing; and a half a dozen other factors.
MaxwellBuchanan, Jun 20 2013
  

       No.
MaxwellBuchanan, Jun 21 2013
  

       //a 10 uL pipette may have three different tips for calibration at 1 uL, 5 uL, and 10 uL//   

       What is ul?   

       /the strange behavior of fluid at that volume/ Yes. Probably there is a better way to move tiny amounts of fluid than a pipette.
bungston, Jun 21 2013
  

       //uL//...//What is ul?//   

       Should be µl - microlitres.
MaxwellBuchanan, Jun 21 2013
  

       What does meniscus formation do to your measurement? Are you still planning to have a specific calibration fluid?
MechE, Jun 21 2013
  

       The meniscus problem is why you would want a long, thin tip rather than a regular conical tip. If the tip is polypropylene (as most are), water will form only a very slight positive meniscus.   

       Moreovermore, before these new-fangled Gilsons, a micropipette was a small glass capillary with a line indicating a defined fill volume. These were used in conjunction with a "spit tube" (capillary holder with tube and mouthpiece) or, since health and safety was invented, a little eyedropper-style bulb. You can still get these, in volumes from 1µl to a few hundred µl.
MaxwellBuchanan, Jun 21 2013
  

       //Should be µl - microlitres//   

       Thanks. I guess mcl was already taken...   

       MB, spent more time than I care to think about with a tiny pipette between my fingers, like some sort of horrible cramping drug addiction. very reliable so long as you take care about where your tears fall.
WcW, Jun 23 2013
  

       The best micropipette calibration tip is "±10% is fine for biology".
MaxwellBuchanan, Jun 24 2013
  
      
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